蛋白酶K說(shuō)明書(shū)

產(chǎn)品簡(jiǎn)介：

  本產(chǎn)品來(lái)源于布氏白僵菌（Beauveria brongniartii）的蛋白酶K基因，經(jīng)過(guò)了基因定點(diǎn)突變和色譜純化。該酶切割脂族氨基酸和芳香族氨基酸的羧基端肽鍵，可以用于所有蛋白質(zhì)的降解。此酶經(jīng)純化去除了RNA酶和DNA酶活性。蛋白酶K是現(xiàn)有蛋白酶中活性最高的品種，在pH4到pH12.5范圍均有活性，反應(yīng)溫度介于0~75℃之間。該蛋白酶K在尿素和SDS中仍然具有降解天然蛋白質(zhì)的能力，主要的應(yīng)用行業(yè)包括：醫(yī)療及基礎(chǔ)研究領(lǐng)域：基因診斷試劑盒；基因組DNA提取試劑盒、RNA提取試劑盒中去除DNA和RNA制備中的核酸酶；制備脈沖電泳的染色體DNA；提取組織中非蛋白成分時(shí)，降解含有蛋白質(zhì)雜質(zhì)；此外，還應(yīng)用于：皮革工業(yè)（皮革軟化及精細(xì)化、脫毛和軟化、蠶絲脫膠），食品、釀造工業(yè)（肉類嫩化、酒類澄清）及洗滌工業(yè)（酶洗衣粉、洗衣液等）。

具體參數(shù)：

來(lái)源種屬：布氏白僵菌（Beauveria brongniartii）

固體酶比酶活：≥30 U/mg

分子量：28 KDa

產(chǎn)品性狀：固體酶為白色凍干粉

純  度：≥95%（SDS-PAGE）

貯存和使用：

固體酶可室溫運(yùn)輸，長(zhǎng)期貯存溫度需保持4℃以下。正確的貯存條件下，有效期為三年。

打開(kāi)包裝后，請(qǐng)及時(shí)使用，以防止吸潮。固體酶溶解后，建議過(guò)濾除菌，分裝為適當(dāng)體積，-20℃貯存。

質(zhì)量控制：

 Proteinase K specification

Product introduction:

  Proteinase K exhibits broad substrate specificity. It degrades many proteins in the native state even in the presence of detergents. Proteinase K is isolated from a fungus, Beauveria brongniartii which is able to grow on keratin. The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. Proteinase K is a stable and highly reactive serine protease. It is stable in a broad range of environments: pH (4 to 12.5), buffer salts, detergents (0.1-0.5% SDS) and temperature (0 to75℃).Proteinase K is commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid. It is also used in leather industry (leather softening, leather refinement, leather depilation and silk degumming), food industry (meat tenderization and wine clarification) and detergent industry. It is highly suited to this application since the enzyme is active in the presence of chemicals that denature proteins, such as SDS and urea, chelating agents such as EDTA, sulfhydryl reagents, as well as trypsin or chymotrypsin inhibitors. Proteinase K is used for the destruction of proteins in cell lysates (tissue, cell culture cells) and for the release of nucleic acids, since it very effectively inactivates DNases and RNases.

Physical Properties:

Appearance (Color): White to Off White

Appearance (Form): Powder

Product name: Proteinase K from Beauveria brongniartii

Purity: 95% (SDS-PAGE)

Units/mg protein: ≥30 U/mg (Enzymatic Activity Unit Definition: One unit will hydrolyze urea denatured hemoglobin to produce color equivalent to 1.0 micromole of tyrosine per minute at pH 7.5 at 37 ℃)

Storage and use:

Long-term storage temperature: -20℃, or not more than 4℃.

The enzyme powder can be transported at room temperature, for short periods.

Under proper storage conditions, the recommended retest period is three years.

地    址：   合肥市經(jīng)濟(jì)技術(shù)開(kāi)發(fā)區(qū)芙蓉路標(biāo)準(zhǔn)廠房1棟B座